Tolerance of Droplet-Digital PCR vs Real-Time Quantitative PCR to Inhibitory Substances
نویسندگان
چکیده
منابع مشابه
Tolerance of droplet-digital PCR vs real-time quantitative PCR to inhibitory substances.
Employment or Leadership: C.R.L. Teo, National University of Singapore; S.S. Chong, National University of Singapore. Consultant or Advisory Role: None declared. Stock Ownership: None declared. Honoraria: None declared. Research Funding: C.G. Lee, National Medical Research Council; S.S. Chong, National Medical Research Council. Expert Testimony: None declared. Patents: C.R.L. Teo, patent number...
متن کاملComparison of droplet digital PCR to real-time PCR for quantitative detection of cytomegalovirus.
Quantitative real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, but a lack of standardization and relatively poor precision have hindered its usefulness. Digital PCR offers highly precise, direct quantification without requiring a calibration curve. Performance characteristics of real-time PCR were compared to those of droplet digital PCR (ddPCR) for cytomegalo...
متن کاملComparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis
Two different quantitative PCR platforms, droplet digital PCR (dd-PCR) and quantitative real-time PCR (qPCR), were compared in a mcrA-based methanogen community assay that quantifies ten methanogen sub-groups. Both technologies exhibited similar PCR efficiencies over at least four orders of magnitude and the same lower limits of detection (8 copies μL-DNA extract-1). The mcrA-based methanogen c...
متن کاملQuantitative Real-Time PCR
Unlike classical end-point analysis PCR, real-time PCR provides the data required for quantification of the target nucleic acid. The results can be expressed in absolute terms by reference to external quantified standards or in relative terms compared to another target sequence present within the sample. Absolute quantification requires that the efficiency of the amplification reaction is the s...
متن کاملReal time quantitative PCR.
We have developed a novel "real time" quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over conta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2013
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2013.211045